p90x workout schedule Phytase Producing Strain and

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Phytase Producing Strain and optimization of solid-state fermentation conditions

. Solid-state fermentation is finished, extracted with buffer, and then use gauze filtration, centrifugation, determination of activity in the supernatant. Generally extracted three times that phytase can be extracted completely,[link widoczny dla zalogowanych], the measured activity of this article were first extracted from each test tube from each of lml, and then 2ml of distilled water were added to enzyme solution, and all kinds of music are without drying, so the higher water content. Table 2 The effect of water on the phytase activity of water: bran 0.8:10.9:11:11.1:11.2:11.3:11.4:1 OD value (60nIn) 0.790.8530 .7830.7670.6800.340.315 8.3549.0068.2818.1167.2163.6963.437 activity can be seen from Table 2, the ratio is 0.9 in the highest activity. Points are too high or too low, the growth of Aspergillus niger, enzyme activity decreased. 2.2.2 Activity of incubation temperature on the production of water can be seen from Table 4, high levels of training activity for 96 hours. 2.2.4 The medium on the activity of glucose. 2.2.2.1 to take 18 500 ml flask, add 12.5 grams of each bottle of wheat bran and 11.25 g distilled water, divided into 6 groups, each group 3. 2.2.2.2121oC sterilization for 30 minutes, cooled vaccination. 2.2.2.3 the 6 groups at 28 ℃, 29 ℃, 30 ℃, 31 ℃, 32 ℃,[link widoczny dla zalogowanych], 33 ~ C incubation temperature for 96 hours. 2.2.2.496 hours later, each bottle of 2% CaC12 solution plus 100 ml extraction flask phytase 1.5 hours. 2.2.2.5 filtered with filter paper and the determination of enzyme activity obtained. Incubation temperature on the production of enzyme activities in Table 3. Table 3 The effect of temperature on phytase activity temperature ℃ 282930313233OD value (60nIn) 0.6010.6990.89r70.6830.6140.592 training activity 6.3987.2059.4627.2476.5326.3042.2.3 the impact of time on enzyme production 2.2.3.1 18 500 ml flask to take a bottle add 12.5 grams of the bran and 11.25 grams of distilled water (1:0.9), divided into 6 groups, each Section 3. 2.2.3.2121oC sterilization for 30 minutes, cooled and inoculated. 2.2.3.3 the 6 groups were cultured 72h, 84h,[link widoczny dla zalogowanych], 96h, 108h, 120h, 132h. 2.2.3.4 to the time, respectively 100 ml of 2% CaC12 solution extraction 1.5 hours, have enzyme. Enzyme activity assay results in Table 4. Table 4 The incubation time of the phytase activity of culture temperature ℃ 303030303030 incubation time h728496108120132OD value (60nIn) 0.6780.7980.8960.8240.65l0.547 activity 7.1958.4379.4528.7066.9155.8392 .2.4.1 take 21 500 ml flask, add 12.5 grams of each bottle of 12.5 grams of wheat bran and distilled water, divided into 7 groups, each group 3. 2.2.4.2 Add the 7 groups were 0%, 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% glucose. 2.2.4.3121oC sterilization for 30 minutes, cooled vaccination. 2.2.4.430 ~ C 4-day incubation, the flask every 12 hours during the period. 2.2.4.54 days later with 100 ml of extraction solution of 2% CaCI2 1.5 hours, had enzyme. Enzyme activity assay results in Table 5. Table 5 glucose on phytase activity of glucose content% OO. 51. O1.52. O2.53, OOD value (6om) O. 895O. 877O-8340.8130.771O. 9l30.732 9.4419.2558.8108.5926.7089.6287.754 activity can be seen from Table 4, the glucose content of 2.5% in the highest activity. When more than 2.5%, the permeability may be a small, low activity. When the glucose content of less than 2.5%, because provides a carbon source, good ventilation, which will help the growth of Aspergillus niger, enzyme activity is relatively high. 3 Conclusion UV, EMS mutagenesis,[link widoczny dla zalogowanych], we get a solid training complex phytase activity was 63.8U / g dry koji ideal strain. Solid state culture conditions for optimum enzyme production: 90% water addition, the optimum temperature for enzyme production was 30 ~ C, the optimal concentration of glucose 2.5%, the best culture period of 96 hours.
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