tory burch sale 氯沙坦&#23

defgf10aj · Wysłany: Śro 11:49, 16 Mar 2011 Temat postu: tory burch sale 氯沙坦&#23

氯沙坦对肝硬化大鼠血清内毒素及一氧化氮的影响
ssi0n0ftransforminggr0wthfactor—plinhumanrenaltubularep—ithelialcellcuIturedwithhighconcentrationofD—glucoseIIUYU—Fang，LIXing(TheSecondH0spitalofShanxiMedicalUniversity，TaiyuanO3OOO1，China)Abstract0bjective：Toinvestigatetheeffectofadip0nectin(ADPN)ontheexpressionoftransforminggrowthfactor—p1(TGF—p1)inhumanrenaltubu1arepithelialcel1culturedwithhighconcentrationofD—g1uc0se．Methods：Hu—manrenaltubularepithelialcellswerecuituredinmediacontaininghighc0ncentrati0nofD—glucoseoradip0nectin．TheexpressionofTGF—p】wasmeasuredbyenzyme—linkedimmunosorbentassay(ELISA)atthe48h．Resu1ts：Theex—pressionofTGF—Blwasprom0tedinhighg1ucosegroup．C0mparedwithhighglucosegroup，theexpressionofTGF—p1wassuppressedinmoderateconcentrati0nadiponectin(1O．O，15．O，2O．Og／mL)．Conc1usi0n：IⅥoderateconcentrationadiponectincandecreasetheexpressionofTGF—p1．Keywordsadip0nectin；humanrena1tubularepithelialcen；transforminggrowthfact0卜B1近年来,[link widoczny dla zalogowanych]，糖尿病肾病(DN)已成为糖尿病患者死亡的主要原因，而在其发生及发展过程中肾小管病变的研究渐为人们所重视。转化生长因子一B(TGF—p)在糖尿病肾病发病机制中起着重要作用。脂联素(ADPN)是近年发现的具有对糖尿病有保护作用的蛋白质，本研究旨在探讨高糖环境下ADPN对人肾小管上皮细胞TGF—B表达的影响，以揭示ADPN对高糖环境下肾小管上皮细胞的可能保护机制。1资料与方法1．1人肾小管上皮细胞的培养与处理人肾小管上皮细胞株培养于含10胎牛血清的低糖DMEM培养液中，置于含5CO浓度、37C恒温培养箱中孵育,[link widoczny dla zalogowanych]，每3～4d用O．25胰酶消化传代,[link widoczny dla zalogowanych]，生长至85融合时接种于96孔细胞培养板，接种密度为1×1O个／mL，待细胞贴壁后，改用无血清DMEM培养液继续培养24h使细胞同步化后，弃去旧的培养液后用于下一步实验。1．2TGF一口表达的检测1．2．1实验分组正常对照组(5．5mmol／L葡萄糖，N组)，高糖组(3O．Ommol／L葡萄糖，G组)，ADPN组(3O．Ommol／L葡萄糖+ADPN,[link widoczny dla zalogowanych]，A组),[link widoczny dla zalogowanych]，ADPN终浓度分别为2．5，5．O，7．5，1OO，15．O，2O．Og／mL，每组设3个复孔，同时设调零孔，培育48h后收集细胞培养上清液进行ELISA检测。1．2．2ELISA检’钡4TGF—B采用由RapidBioLab．Calabasas，CaIifornia，USA提供
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