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Hibiscus anti-flu tablets Determination of total flavonoids

To 6.0ml, precision of 5% sodium nitrite solution added 1.0ml, shake , place 6min. Precision adding 375 9 No. 4 April 2003 , Hebei Medical HEB Ⅱ MEDICINEVoi. 9, No. 4Apt. , 2003lO% aluminum nitrate solution 1. O Ⅱ d,[link widoczny dla zalogowanych], shake , place 6min, precision test solution by adding sodium hydroxide 10. O Ⅱ d, shake, add water to the mark , place 15min, ethanol as a blank , by UV-visible spectrophotometric determination of absorbance at 500nm wavelength . The concentration of C (/ zg.ml) for the vertical axis , absorbance A as abscissa , the standard curve , the regression equation was C = 77.089A + O. 299l, the correlation coefficient r = 0.999. Line range is 200.01200 . O. ml -1 . 2.2 Determination of Sample : Take 20 to sugar , said that given weight, grind fine , take the powder about 0.36g, accurately weighed, ultrasonic extraction with ethanol 2 times 30min, 10mlf Times. Filtered to 25 Ⅱ d flask washed using ethanol and diluted to the mark , shake, precision drawing 2.0ml set 25 Ⅱ d flask ,[link widoczny dla zalogowanych], add ethanol to the 6.0nd, precision in 5% sodium nitrite solution 1.0nd, shake, Place 6min, 10% precision aluminum nitrate solution by adding 1. O Ⅱ d, shake , place 6min, precision test solution by adding sodium hydroxide 10.0ml, shake, add water to the mark , place 15min, ethanol as a blank , by UV-visible spectrophotometric determination of absorbance at 500nm wavelength . The results in Table l. Hibiscus Table 1 assay results against silicon reference substance by adding appropriate amount of rutin , according to the method of determination , calculation rate ,[link widoczny dla zalogowanych], the results in Table 2. Table 2, recoveries of rutin sample test results Note: The addition of test reproducibility are 0.340mg2.4 : take 5 copies of the same batch of samples , according to the parallel determination of 5 times , the result was 0.78% RSD (n = 5). 2.5 Stability Test : Take the same sample , the absorbance measured lh each ,[link widoczny dla zalogowanych], the result RSD was 0.75% (n = 5). 3 discuss the use of UV- visible spectrophotometry of total flavonoids content, method is stable , accurate and can be used for the quality control methods.
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