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byt ∞ mailataAna1. Biechem. 1981,111:372 ~ 375 [6] ToteSS, ChrkNThee ~ feetofL-methJonlneeoneentration ∞ juveai] ehormonebiosynthesiyeor0oraallataofthecock-roach, l ~ plopterapunctataInsectBiceheTa, 1985t15: 175 ~ 179 [7] (rNA, JanWPInhibitionofMamtucaco'l ~ oraailatain ~ dtrohyacerehralailato ~ tatieneuropepfideMolCeLlEndocrinoi,[link widoczny dla zalogowanych], 1987 ,[link widoczny dla zalogowanych], 49:237 ~ 248 [8] Kau ~ kaH,[link widoczny dla zalogowanych], A, LiJPegalIdentificationof ∞ athtotropinfromaduLtMand ~ sextaScleace.1989.243: 1481 insects of 43 volumes PreliminaryisolationandpurificationofallatostatinfromMythimnaseparataOUYANGYing · chun, TANGShuang, GUANXue · chen,[link widoczny dla zalogowanych], FANGYuding (staKeyLaboratorydImegratedM Rights ag coffee ntdPtlva,[link widoczny dla zalogowanych], eet ~ andRode port dock, I adjoin bared Zoc2egytheChineseAcademyofSclenoes, Bering100080, China) Abstract: Inthisreport, aprehminaryisolationofASfromthebrainsoflarvae, Mythimnaseparata, isdescribed.TheAS-activitymaterialwhichcaninhibitJHsynthesiyCAOCCLLrSinthebrainsoflastin-stsxTheabilityofbrainextracttoinhibitJHsynthesisinvitrowastestedbyradiochemicaIassayUsing1brainequivalent, 51% inhlbitionofJHsynthesiswasachieved.Ifthebrainextractmpretreatedwithtrypsin, theinhibitoryeffectoftheextractwasgreatlyreducedAssaysoffractionsfromthereversabphaseC1Rcolumnof} jingling LCseparationshowedthatmaterialswithASactivitywereelutedbetween5.2% to28% methanoI (fraction1 ~ 20) , andbet% ween40% to76% methanoI (fraction30 ~ 60) Assaysoffractions3, 5,11,40,54 and60using5brainequivalentsperpairofCAshowed> 50% inhibitionofJHsynthe · sis.FurtherpurificationofthefractionsfromtheCcolumnwithallatostaticactivityiSinprogress.Keywords: Mythimnaseparata; allatostatin; brainextract; HPLC
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